CXCL13-CXCR5 is responsible for ZNF382 down-regulation–induced neuropathic pain . (A–C) Effect of premicroinjection with Cxcl13 siRNA (siCXCL13) or scrambled siRNA (Scr) into the ipsilateral L4 DRG on the development of SNL-induced mechanical allodynia (A and B) and heat hyperalgesia (C). n = 12 mice/group. Data from two independent experiments. Two-way RM ANOVA followed by the post hoc Tukey test. **, P < 0.01; ***, P < 0.001 versus SNL plus Scr group. (D–F) Effect of post-microinjection with CXCL13 antibody (CXCL13 Ab) or NC IgG into the ipsilateral L4 DRG on the maintenance of SNL-induced mechanical allodynia (D and E) and heat hyperalgesia (F). n = 8 mice/group. Data from two independent experiments. Two-way RM ANOVA followed by the post hoc Bonferroni’s test. *, P < 0.05; **, P < 0.01 versus SNL plus NC group. (G–I) Effect of microinjection with CXCL13 or vehicle into the ipsilateral L3/L4 DRGs of naive mice on paw withdrawal responses to mechanical (G and H) and heat (I) stimuli. n = 8 mice/group. Data from two independent experiments. Two-way RM ANOVA followed by post hoc Bonferroni’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001 versus the vehicle group. BL, baseline. (J–L) Microinjection of Cxcl13 siRNA (siCXCL13; dashed arrow) into the ipsilateral L3/L4 DRGs attenuated AAV5-Znf382 shRNA (shRNA)–induced mechanical allodynia (J and K) and heat hyperalgesia (L). n = 12 mice/group. Data from two independent experiments. Two-way RM ANOVA followed by post hoc Bonferroni’s test. *, P < 0.05; **, P < 0.01 versus the shRNA plus Scr group. BL, baseline. (M–O) Effect of microinjection with AAV5-Znf382 shRNA (shRNA) or AAV5-scrambled shRNA (Scr) into the ipsilateral L3/L4 DRGs of WT or Cxcr5^−/− mice on paw withdrawal responses to mechanical (M and N) and heat (O) stimuli. n = 8 mice/group. Two-way RM ANOVA followed by post hoc Tukey test. *, P < 0.05; **, P < 0.01 versus the WT plus shRNA group.