S1PR5 promotes T cell relocation to vascular beds. (A) P14 T cells were transduced with control (Ctrl)-RV or S1PR5-RV and transferred into LCMV-infected mice. Transduced cells were sorted from the spleen 8 dpi. Expression of S1pr5 was quantified by qPCR and normalized to a housekeeping gene. (B–D) OT-I T cells were transduced with Ctrl-RV or S1PR5-RV and cotransferred i.v. into mice treated with DNFB. Mice received anti-CD45 antibody i.v. to label vasculature-associated cells before harvest. Shown are numbers (B) and frequencies (C) of Ctrl-RV and S1PR5-RV cells isolated from the indicated organs 8 d after transfer and the Bcl2/Bim ratio (left) and percentage annexin+ live/dead+ (L/D) apoptotic cells (right) of Ctrl-RV and S1PR5-RV cells isolated from the indicated organs 8 d after transfer (D). In A, data are pooled from two independent experiments, with n = 3 mice per group per experiment. In B–D, data are representative of two independent experiments, with n = 5–10 mice per experiment. Multiple paired t test was used in B and multiple unpaired t test in C and D. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Graph shows mean ± SD. A.U., arbitrary units; TEFF, effector T cell.