Figure S1.
KLF2 does not directly regulate S1pr5 expression. (A and B) Effector P14 T cells were nucleofected with control Cd19-targeting (sgCd19), Klf2-targeting (sgKlf2), or Tbx21-targeting (sgTbx21) sgRNA/Cas9 RNPs and were maintained in culture for 3 d. (B) Effector P14 T cells were transduced with a control (Ctrl-RV) or a KLF2 (KLF2-RV) R (RVs) before nucleofection with sgRNA/Cas9 RNPs. Expression of the indicated genes was quantified by qPCR. In A and B, data are pooled from two independent experiments, with n = 2–6 samples per condition. Multiple Mann–Whitney test was used in A and multiple t test in B. n.s., nonsignificant; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. rel., relative; TEFF, effector T cell.

KLF2 does not directly regulate S1pr5 expression. (A and B) Effector P14 T cells were nucleofected with control Cd19-targeting (sgCd19), Klf2-targeting (sgKlf2), or Tbx21-targeting (sgTbx21) sgRNA/Cas9 RNPs and were maintained in culture for 3 d. (B) Effector P14 T cells were transduced with a control (Ctrl-RV) or a KLF2 (KLF2-RV) R (RVs) before nucleofection with sgRNA/Cas9 RNPs. Expression of the indicated genes was quantified by qPCR. In A and B, data are pooled from two independent experiments, with n = 2–6 samples per condition. Multiple Mann–Whitney test was used in A and multiple t test in B. n.s., nonsignificant; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. rel., relative; TEFF, effector T cell.

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