IRSp53-dependent phase separation of the PSD complex. (A) Sedimentation experiments showing the phase separation capability of PSD components when 4× PSD (PSD-95, GKAP, Shank3, and Homer3) was mixed IRSp53 (WT and mutant) at 5 μM. Distributions of each PSD component are quantified and shown as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001 using one-way ANOVA with Dunnett’s multiple comparison test. (B) Sedimentation experiments showing the phase separation capability of PSD components when 4× PSD* (PSD-95, GKAP, Shank3_ΔPRM, and Homer3) was mixed with IRSp53 (WT and mutant) at 5 μM. Distributions of each PSD component are quantified and shown as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 using one-way ANOVA with Dunnett’s multiple comparison test. Source data are available for this figure: SourceData FS3.
Figure S3.

IRSp53-dependent phase separation of the PSD complex. (A) Sedimentation experiments showing the phase separation capability of PSD components when 4× PSD (PSD-95, GKAP, Shank3, and Homer3) was mixed IRSp53 (WT and mutant) at 5 μM. Distributions of each PSD component are quantified and shown as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001 using one-way ANOVA with Dunnett’s multiple comparison test. (B) Sedimentation experiments showing the phase separation capability of PSD components when 4× PSD* (PSD-95, GKAP, Shank3_ΔPRM, and Homer3) was mixed with IRSp53 (WT and mutant) at 5 μM. Distributions of each PSD component are quantified and shown as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 using one-way ANOVA with Dunnett’s multiple comparison test. Source data are available for this figure: SourceData FS3.

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