Tacrine hydrochloride inhibits CCA with aberrant CLK3 expression. (A and B) Tacrine hydrochloride inhibited the EGF-induced increase in CLK3 expression, USP13 phosphorylation at Y708 (A), and proliferation in HCCC9810 and HuH-28 cells (B). (C) Measuring the levels of purine in HuCCT1, HuH-28, and HiBEC cells treated as indicated. (D and E) The effect of tacrine hydrochloride on the growth (D) and migration (E, i) of HuCCT1, HuH-28, and HiBEC cells and on the invasion (Eii) of HuCCT1 and HuH-28 cells. *, P < 0.05; **, P < 0.01; ***, P < 0.001; #, not significant. Data are mean ± SEM and are from three (B–E) independent experiments or are representative of three independent experiments with similar results (A). P values were calculated using unpaired Student’s t test (B–E). TUBA, alpha tubulin.
Figure S5.

Tacrine hydrochloride inhibits CCA with aberrant CLK3 expression. (A and B) Tacrine hydrochloride inhibited the EGF-induced increase in CLK3 expression, USP13 phosphorylation at Y708 (A), and proliferation in HCCC9810 and HuH-28 cells (B). (C) Measuring the levels of purine in HuCCT1, HuH-28, and HiBEC cells treated as indicated. (D and E) The effect of tacrine hydrochloride on the growth (D) and migration (E, i) of HuCCT1, HuH-28, and HiBEC cells and on the invasion (Eii) of HuCCT1 and HuH-28 cells. *, P < 0.05; **, P < 0.01; ***, P < 0.001; #, not significant. Data are mean ± SEM and are from three (B–E) independent experiments or are representative of three independent experiments with similar results (A). P values were calculated using unpaired Student’s t test (B–E). TUBA, alpha tubulin.

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