Cilia assembly and disassembly in HEK293T cells and the localization of CCT proteins in CEP55-depleted cells, related to Fig. 6. (A) HEK293T cells were starved for 48 h. Ciliated cells were then stimulated with serum for 18 h and stained with ARL13B (green) and DNA (blue). Scale bar, 10 µm. (B) Quantification of the ciliated cells in A. Data are presented as means ± SD of three independent experiments. A one-way ANOVA test was performed. ***, P < 0.001. n, number of cells. (C–F) RPE-1 cells transfected with control or CEP55 siRNA were stained with CCT proteins (green), γ-tubulin (red), and DNA (blue; CCT1 in C, CCT2 in D, CCT5 in E, and CCT8 in F). Insets show zoomed-in views of the boxed regions. Scale bars, 5 µm (main image) and 1 µm (magnified region).