Cep55 deficiency inhibits cilia disassembly and the stability of Aurora A protein in primary MEFs, related to Fig. 5. (A) Cep55^+/+ and Cep55^−/− primary MEFs were starved for 48 h. The ciliated primary MEFs were then stimulated with serum for the indicated times and stained with Ac-tubulin (green) and DNA (blue). Scale bar, 10 µm. (B) Cell lysates in A were detected with the indicated antibodies. (C) Immunoblot analysis with anti-Cep55 and anti–Aurora A antibody on protein extracts from E9.5 Cep55^+/+, Cep55^+/−, and Cep55^−/− neural tubes. GAPDH was used as a loading control.