CEP55 interacts with Aurora A and localizes at the centrosome, related to Fig. 4. (A) Eluates from representative Flag-vector and Flag-CEP55 immunoaffinity purification experiments were stained with Coomassie blue and subjected to mass spectrometric sequencing. M, marker. (B) CEP55 (green) and Aurora A (red) were costained with a centriolar distal marker (centrin-2, purple) and DNA (blue). Insets show zoomed-in views of the boxed regions. Scale bars, 5 µm (main image) and 1 µm (magnified region). (C) RPE-1 cells were starved for 48 h. The ciliated cells were then stimulated with serum for 2 h and stained with CEP55 (green), p-Aurora A (red), Ac-tubulin (gray), and DNA (blue). Insets show zoomed-in views of the boxed regions. Scale bars, 5 µm (main image) and 0.5 µm (magnified region).