CEP55 is dispensable for the centrosome integrity and its localization in different cell cycle stages, related to Fig. 3. (A–D) RPE-1 cells were transfected with control or CEP55 siRNA in the presence of serum and then stained with indicated antibodies. The localization of γ-tubulin (A, red), Centrin-2 (B−D, green), Cep164 (C, red), and PCM1 (D, red) was not affected by CEP55 depletion. Insets show zoomed-in views of the boxed regions. Scale bars, 5 µm (main image) and 1 µm (magnified region). (E) A table summarizing the centrosome localization of the tested proteins in CEP55-depleted RPE-1 cells. (F–H) Asynchronously growing RPE-1 cells were stained with endogenous CEP55 (green), γ-tubulin (red), and DNA (blue). Representative cells from different stages of the cell cycle are shown. Insets show zoomed-in views of the boxed regions. Scale bars in F and G, 5 µm (main image) and 1 µm (magnified region). Scale bars in H, 10 µm (main image) and 1 µm (magnified region).