Whi8 binds and recruits the CLN3 mRNA to SGs. (A) MCP-NLS-GFP foci numbers in WHI8-mCh cells (n = 200) with plasmids expressing either CLN3 or CLN3-MS2v6 after 30 min at 42°C in the absence of glucose. (B)PUB1-mCh cells with the indicated genotypes with plasmids expressing MCP-NLS-GFP and CLN3-MS2v6 were stressed for 30 min at 42°C in the absence of glucose. Foci detected above a fixed local threshold with the aid of BudJ are also shown. Scale bar, 2 µm. wt, wild type.