CDKA;1 colocalizes with the spindle in metaphase I and metaphase II. (A) Confocal laser scanning micrographs showing the localization of TagRFP:TUA5 (magenta, first column) expressed from the PRO_RPS5A promoter and mVenus (green, second column) expressed from the PRO_CDKA;1 promoter in late prophase, shortly before NEB, metaphase I, and metaphase II. Third column represents the merge of the two channels. (C) Signal intensity plot profile of a section (white line) through one meiocyte shown in A (close-up in second column). (B) Confocal laser scanning micrographs showing the localization of Tag:RFP:TUA5 (magenta, first column) expressed from the PRO_RPS5A promoter and a CDKA:mVenus (green, second column) fusion proteins expressed from the PRO_CDKA;1 promoter in late prophase, shortly before NEB, and metaphase I and II. Third column represents the merge of the two channels. (D) Signal intensity plot profile of a section (white line) through one meiocyte (close-up in second column). Scale bar, 10 µm.