An immobilized mitochondrial network abrogates mrps-5 RNAi-mediated lifespan extension. (A) Survival curves performed in N2 and drp-1(tm1108) demonstrating that mutation of drp-1 significantly enhances mrps-5 RNAi-mediated longevity. See Table S1 for lifespan statistics. (B) Lifespan analysis of N2 revealing the pro-longevity effects of mrps-5 RNAi. Survival curves were compared using the log-rank test. See Table S1 for lifespan statistics. (C) Lifespan analysis of drp-1;fzo-1 double mutant showing that combined mutation of fzo-1 and drp-1 abrogates mrps-5 RNAi-mediated lifespan extension. Survival curves were compared using the log-rank test. See Table S1 for lifespan statistics. (D) The transcript level of hsp-6 in day 1 adult N2, drp-1(tm1108), and drp-1;fzo-1 mutant upon mrps-5 RNAi. The expression levels of hsp-6 were normalized to reference genes ama-1, cdc-42, and F35G12.2, and compared with the mean value of ev-treated N2 worms. The results were derived from two independent experiments. Differences were determined by Student’s t test; *, P < 0.5; **, P < 0.01. (E) Mitochondrial networks in muscle cells of drp-1;fzo-1 double mutant upon mrps-5 RNAi. Animals were examined at day 2 of adulthood. Scale bar in ev-treated condition is 10 µm and valid for all the images in E. (F) Quantification of mitochondrial morphologies in E: mean ± SEM of 33–42 different animals, pooled from two independent experiments. ns, not significant by Student’s t test.