CLASP2 KT localization and MT-binding properties are essential for chromosome segregation fidelity. (A) Immunofluorescence analysis of the different U2OS PA–GFP–α-tubulin cell lines in anaphase. Segregation errors are indicated with red arrows in the DAPI channel. Parental and CLASP2 mutant cell lines depleted of endogenous CLASPs showed increased chromosome segregation errors relative to WT. Scale bar is 5 µm. (B) Quantification of chromosome segregation errors in anaphase and telophase cells. The first column represents parental U2OS cells without RNAi treatment; the remaining columns represent treatment with the siRNA #12A. Quantifications from a pool of three independent experiments: Parental = 92 cells; Parental with RNAi = 117 cells; WT = 153 cells; 2ea-3eeaa = 126 cells; IP12 = 135 cells; 2ea-IP12 = 53 cells; IP12-3eeaa = 121 cells; ΔC = 120 cells; ΔC-Gcn4 = 106 cells; ΔC-Spc25 = 86 cells; C-term = 73 cells. *, P < 0.05; **, P < 0.01; ***, P < 0.001; t test.