Figure 3. Regulation of mitotic spindle length by CLASP2 depends on its KT localization and MT-binding properties. (A) Immunofluorescence analysis of spindle length in the different U2OS PA–GFP–α-tubulin cell lines in metaphase. Scale bar is 5 µm. (B) Quantification of mitotic spindle length in each cell line. The first column represents the parental U2OS cell line and the remaining columns represent treatment with siRNA #12A. Each data point represents an individual cell. Bars represent mean and SD. Quantifications from a pool of three independent experiments: Parental = 88 cells; Parental with RNAi = 74 cells; WT = 108 cells; 2ea-3eeaa = 96 cells; IP12 = 105 cells; 2ea-IP12 = 82 cells; IP12-3eeaa = 88 cells; ΔC = 112 cells; ΔC-Gcn4 = 142 cells; ΔC-Spc25 = 113 cells; C-term = 123 cells. ***, P < 0.001; ****, P < 0.0001; t test.
Figure 3.

Regulation of mitotic spindle length by CLASP2 depends on its KT localization and MT-binding properties. (A) Immunofluorescence analysis of spindle length in the different U2OS PA–GFP–α-tubulin cell lines in metaphase. Scale bar is 5 µm. (B) Quantification of mitotic spindle length in each cell line. The first column represents the parental U2OS cell line and the remaining columns represent treatment with siRNA #12A. Each data point represents an individual cell. Bars represent mean and SD. Quantifications from a pool of three independent experiments: Parental = 88 cells; Parental with RNAi = 74 cells; WT = 108 cells; 2ea-3eeaa = 96 cells; IP12 = 105 cells; 2ea-IP12 = 82 cells; IP12-3eeaa = 88 cells; ΔC = 112 cells; ΔC-Gcn4 = 142 cells; ΔC-Spc25 = 113 cells; C-term = 123 cells. ***, P < 0.001; ****, P < 0.0001; t test.

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