Sites of Exo70 mutants within the cryo-EM model for the exocyst complex. Cryo-EM model of the exocyst complex (Mei et al., 2018; PDB accession no. 5YFP), with Exo70 shown in blue and the gain-of-function mutants indicated as yellow spheres. Top: The Exo70 subunit is shown in isolation, with domains A–D specifically labeled in relation to the position of the I114F, G388R, and D541Y point mutations. Insets show zoomed-in views of the close apposition of the Exo70 mutant residues with Sec6 (purple), Exo84 (pink), Sec15 (red), Sec8 (dark green), and Sec5 (light green). Negative stain data indicates increased flexibility throughout the entire A–D region of Exo70, with concomitant effects on Sec6.