Strip and Cka control Slik localization and function in the wing disc epithelium. (A–C) Confocal projections through medial regions of wing discs expressing GFP (green) in a stripe of cells under control of the ptc-GAL4 driver, either alone (A) or together with dsRNA targeting Strip (B) or Cka cDNA (blue) and p-moesin (red). Arrows indicate downregulation of p-moesin levels in dSTRIPAK-depleted cells in the wing hinge region. Images in A′–C′ show higher-magnification projections of the hinge region. Yellow dotted lines indicate limits of the GFP-transgene expression domains. (D–F) Quantification of normalized p-moesin fluorescence intensity in hinge cells expressing GFP alone (D) or together with dsRNA targeting Strip (E) or Cka (F) compared with their respective adjacent wild-type (GFP−) cells. Fluorescence was measured at multiple folds in five wing discs for each genotype. Two-tailed paired sample t test. (G–I) Confocal projections through the hinge region of wing discs expressing GFP (green) alone (G) or together with dsRNA targeting Strip (H) or Cka (I). Discs were stained with DAPI (blue) to visualize nuclei and antibody against Slik (red). Yellow dotted lines indicate limits of the GFP-transgene expression domains. (J–O) Quantification of normalized Slik fluorescence intensity in hinge cells as above. Measurements were taken in the apical (J, L, and N) or basal (K, M, and O) halves of the cells at multiple folds in four wing discs for each genotype. Two-tailed paired t-test. P values are as follows: NS, P > 0.05; **, P < 0.01; ***, P < 0.001.