PP2A_STRIPAK regulates Slik cortical association in mitosis. (A and E) Time lapse of Slik distribution in live S2 cell stably expressing control or mutated Slik-GFP (white) and undergoing mitosis. Slik-GFP signals were pseudocolored in a rainbow heatmap to underline variations in its distribution in metaphase (right row). (C) Time lapse of Slik distribution in live S2 cell stably expressing Slik^WT-GFP (white) upon the indicated treatments after the first metaphase time frame. Slik^WT-GFP signal was pseudocolored in a rainbow heatmap to underline variations in its distribution after 20 min of treatment (right row). (B, D, and F) The ratios of the indicated Slik-GFP constructs (cortex/cytoplasm) were quantified and normalized to the first time frame in a minimum of six cells/condition in three independent experiments. Data are represented as normalized mean ± SEM of the pooled experiments, and one-way ANOVA was calculated against the first time frame with the following P values: NS, P > 0.05; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Scale bars, 5 µm.