Slik localization is affected by PP2A_STRIPAK inhibition or kinase inhibition. (A) Slik intensity at the cortex was quantified in a minimum of 60 cells/condition (see Fig. 4 D) in three independent experiments. Data are represented as the mean value ± SD of the pooled replicates (open circles) normalized to the nontarget (NT) dsRNA, one-way ANOVA with the following P values: **, P < 0.01; ****, P < 0.0001. (B) The ratio of Slik intensity (cortex/cytoplasm) was quantified in a minimum of 60 cells/condition (see Fig. 4 F) in three independent experiments. Data are represented as the mean value ± SD of the pooled replicates (open circles) normalized to DMSO treatment, one-way ANOVA with the following P values: **, P < 0.01; ****, P < 0.0001. (C) Time lapse of Slik distribution in live S2 cell stably expressing Slik^WT-GFP (white) upon indicated treatments after the first time frame. (D) The ratio of Slik^WT-GFP intensity (cortex/cytoplasm) was quantified in a minimum of 12 cells/condition in three independent experiments. Fold changes of the ratio of Slik-GFP before and after the treatments (30 min for OA; 60 min for Stau) were quantified (each dot represents the value for a single cell). Data are represented as mean ± SD of the pooled experiment, paired t test with the following P values: NS, P > 0.05; ****, P < 0.0001. (E) The ratio of Slik^WT-GFP (cortex/cytoplasm) was quantified in 40 cells/condition (see Fig. 5 B) in three independent experiments. Data are represented as mean ± SD of the pooled replicates (circles), unpaired t test with the following P values: *, P < 0.05; **, P < 0.01. (F) The ratio of the indicated Slik-GFP constructs (cortex/cytoplasm) was quantified in 30 cells/condition (see Fig. 5 E) in three independent experiments. Data are represented as mean ± SD of the pooled replicates (circles), one-way ANOVA with the following P values: *, P < 0.05; **, P < 0.01.