PP2ASTRIPAK regulates Slik localization at the cell cortex. (A and C) Projection of three confocal planes of S2 cells upon the indicated treatment and stained for Slik (green), F-actin (red), and DNA (blue). Enlarged areas of the cells are shown. (B and D) Slik intensity at the cortex (B) or the ratio of Slik intensity (cortex/cytoplasm; D) in a minimum of 60 cells/condition in three independent experiments (see also Fig. S3). Data are represented as mean ± SD (each dot represents the value for a single cell), one-way ANOVA with the following P value: ****, P < 0.0001. Scale bars, 5 µm and 2 µm for enlarged areas.
Figure 4.

PP2ASTRIPAK regulates Slik localization at the cell cortex. (A and C) Projection of three confocal planes of S2 cells upon the indicated treatment and stained for Slik (green), F-actin (red), and DNA (blue). Enlarged areas of the cells are shown. (B and D) Slik intensity at the cortex (B) or the ratio of Slik intensity (cortex/cytoplasm; D) in a minimum of 60 cells/condition in three independent experiments (see also Fig. S3). Data are represented as mean ± SD (each dot represents the value for a single cell), one-way ANOVA with the following P value: ****, P < 0.0001. Scale bars, 5 µm and 2 µm for enlarged areas.

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