dSTRIPAK phosphatase activity regulates moesin phosphorylation. (A) p-Moesin immunofluorescence intensity in S2 cells treated as indicated and normalized to the nontreated condition. Data are represented as normalized mean ± SEM of two to four independent experiments (circles), and two-tailed unpaired t tests were calculated against the nontreated condition. (B and D) Western blot of S2 cells treated as indicated. (C, E, and F) Relative p-moesin levels and relative moesin levels normalized as indicated in three to eight independent experiments (circles). Data are represented as normalized mean ± SD, one-way ANOVA with the following P values: NS, P > 0.05; *, P < 0.05; **, P < 0.01; ****, P < 0.0001.