Depletion of Mob4, Slmap and FGOP2, three dSTRIPAK adaptor proteins, slightly decrease p-moesin levels. (A) Automated quantification of the immunofluorescence intensity of p-moesin in S2 cells treated as indicated and normalized to nontreated condition. Data are represented as normalized mean ± SEM of two to four independent experiments (circles). Two-tailed unpaired t tests were calculated against the nontreated condition with the following P values: NS, P > 0.05; *, P < 0.05. (B and D) Western blot of S2 cells, treated as indicated, with antibodies against Strip, Cka, Mts, and β-actin or α-tubulin. Knockdown of PP2A-29B destabilizes Mts as previously described in mammalian cells (Strack et al., 2004). Knockdown of Cka destabilizes Strip. (C and E) Relative Strip, Cka, or Mts levels (normalized as indicated) were quantified in two to four independent experiments (circles). Data are represented as mean ± SD normalized on nontarget (NT) dsRNA, one-way ANOVA with the following P values: NS, P > 0.05; **, P < 0.01; ****, P < 0.0001. (F) Relative Strip or Cka mRNA levels (normalized to Act5c and Rpl32) were quantified by quantitative real-time PCR in total RNA extracts of S2 cells treated with indicated dsRNA. (G) Western blot of S2 cells treated as indicated with antibodies against T559-phosphorylated p-moesin, moesin, and β-actin. (H) Relative p-moesin levels (normalized to moesin) were quantified in four independent experiments (circles). Data are represented as mean ± SD normalized on NT dsRNA, one-way ANOVA with the following P values: NS, P > 0.05; **, P < 0.01.