Schematic of fluorescence intensity quantification. (A) Diagram illustrating where fluorescence intensity quantifications were measured. A region of interest was drawn around the nucleus (dashed black line) and each acentric (dashed purple lines). The fluorescence intensity of the nuclear envelope marker was then measured. Values were normalized (see Materials and methods). (B) Theoretical graph depicting the normalized fluorescence intensity on nuclei (black line) and acentrics (purple line) over time. The red box represents the hypothetical values measured from A. See also Figs. 3 and 4.