Figure 2.
CCL2-recruited IMs promote bone metastasis growth.(A) Diagram of adoptive transfer of monocytes in healthy mice and mice with bone metastasis. (B) Relative number of recruited IMs and PMs in bone of recipient mice bearing bone metastasis or healthy controls. n = 6; *, P < 0.05 with Student’s t test. Experiments performed two times independently. (C) Survival curve (defined as time taken to reach the humane end point) of mice with MetBo2 bone metastasis. WT, n = 4; knockout, n = 9; P = 0.0133. (D) Representative images and quantification of bioluminescent (BLI) signal for MetBo2 bone metastasis in Ccr2−/− mice and WT mice. Signals were quantified by PhotonIMAGER and normalized to day 0. Ccr2−/− group, n = 8 mice; WT group, n = 7 mice. Bar represents SEM. **, P < 0.01 with Student’s t test. Experiments performed four times independently. (E) Survival curve of mice with bone metastasis. Death is defined as time the mice take to reach the humane end point. P = 0.0332. (F) Representative images and quantification of bioluminescent signal for 1833 human bone metastasis model in Ccr2−/− mice and WT mice. Signals were measured by IVIS and normalized to day 0. Ccr2−/− group, n = 8 mice; control group, n = 7 mice. Bar represents SEM. ***, P < 0.001 with Student’s t test. (G) Quantification of bioluminescent signal for 1833 human bone metastasis model treated with anti-CCL2 inhibitory antibody (Ab) or corresponding isotype control (IgG). Signals were measured by IVIS and normalized to day 0. CCL2 antibody–treated group, n = 8 mice; control group, n = 7 mice. Bar represents SEM. ***, P < 0.001 with Student’s t test. (H) Diagram and quantification of bioluminescent signal of monoycte add-back. Recipient mice are Ccr2-deficient mice with 1833 bone metastasis. Monocytes from WT mice and Ccr2-deficient mice were injected i.v. after tumor signal was detected. Bioluminescent signals on the legs were quantified by PhotonIMAGER and normalized to day 0. Each group contain data from four legs. Bar represents SEM. ***, P < 0.001 with Student’s t test. n = 4–5. Experiments performed three times independently.

CCL2-recruited IMs promote bone metastasis growth. (A) Diagram of adoptive transfer of monocytes in healthy mice and mice with bone metastasis. (B) Relative number of recruited IMs and PMs in bone of recipient mice bearing bone metastasis or healthy controls. n = 6; *, P < 0.05 with Student’s t test. Experiments performed two times independently. (C) Survival curve (defined as time taken to reach the humane end point) of mice with MetBo2 bone metastasis. WT, n = 4; knockout, n = 9; P = 0.0133. (D) Representative images and quantification of bioluminescent (BLI) signal for MetBo2 bone metastasis in Ccr2−/− mice and WT mice. Signals were quantified by PhotonIMAGER and normalized to day 0. Ccr2−/− group, n = 8 mice; WT group, n = 7 mice. Bar represents SEM. **, P < 0.01 with Student’s t test. Experiments performed four times independently. (E) Survival curve of mice with bone metastasis. Death is defined as time the mice take to reach the humane end point. P = 0.0332. (F) Representative images and quantification of bioluminescent signal for 1833 human bone metastasis model in Ccr2−/− mice and WT mice. Signals were measured by IVIS and normalized to day 0. Ccr2−/− group, n = 8 mice; control group, n = 7 mice. Bar represents SEM. ***, P < 0.001 with Student’s t test. (G) Quantification of bioluminescent signal for 1833 human bone metastasis model treated with anti-CCL2 inhibitory antibody (Ab) or corresponding isotype control (IgG). Signals were measured by IVIS and normalized to day 0. CCL2 antibody–treated group, n = 8 mice; control group, n = 7 mice. Bar represents SEM. ***, P < 0.001 with Student’s t test. (H) Diagram and quantification of bioluminescent signal of monoycte add-back. Recipient mice are Ccr2-deficient mice with 1833 bone metastasis. Monocytes from WT mice and Ccr2-deficient mice were injected i.v. after tumor signal was detected. Bioluminescent signals on the legs were quantified by PhotonIMAGER and normalized to day 0. Each group contain data from four legs. Bar represents SEM. ***, P < 0.001 with Student’s t test. n = 4–5. Experiments performed three times independently.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal