Pharmacologic inhibition of EYA2 Tyr phosphatase suppresses glioblastoma growth. (A and B) Kaplan–Meier survival curves of immunocompromised mice bearing GSC 387 (A) or GSC 3565 (B) treated with benzbromarone or vehicle. The P values were calculated by Mantel-Cox log-rank test. **, P < 0.01; ***, P < 0.001. I.C., intracranial. (C) Bioluminescence images of mice bearing GSC 3565 treated per os with either vehicle or 30 mg/kg 9987. (D) Quantification of the bioluminescence signals from either 9987-treated mice or vehicle-treated mice. Data are presented as mean ± SD. *, P < 0.05. The P values were calculated by two-way ANOVA with Tukey’s correction for multiple comparisons. (E) The weight of tumors dissected from tumor-bearing mice 25 d after tumor cell implantation. Data are presented as mean ± SD. *, P < 0.05. The P values were calculated by unpaired t test. (F and G) The number of spheres generated from either 500 (F) or 100 (G) FACS-sorted tumor cells. Data are presented as mean ± SD. *, P < 0.05. The P values were calculated by unpaired t test. (H–J) mRNA levels of SOX2 (H), OLIG2 (I), and MYC (J) measured by qPCR in tumor cells sorted from either vehicle-treated mice or 9987-treated mice. Data are presented as mean ± SD. *, P < 0.05. The P values were calculated by unpaired t test. 10 mice per arm were used for A and B, and five mice per arm for C–J.