The effect of Ang II on ILC2s is dependent on IL-33. (A–F) IL-33 ^+/+ and IL-33^−/− mice were i.n. administered Ang II or PBS daily for 5 d. Proportions of lung ILC2s in CD45⁺ cells (A), ILC2 proliferation (B), and cytokine production of lung ILC2s (C) were determined by flow cytometry. (D) Percentages of eosinophils in BALF. (E) Concentration of type 2 cytokines in BALF. (F) H&E staining of lung tissues and quantitated histological scoring. (G) Expression of Il33 in lung epithelial cells after Ang II or papain challenge. (H–J) Naive mice were i.p. injected with PBS or Ang II (40 µg/d/mouse) after papain (10 µg/d/mouse) challenge. (H) Intracellular cytokine production by lung ILC2s. (I) Percentages of eosinophils. (J) H&E staining of lung sections and quantitated histological scoring (scale bar, 100 µm). All data are from two or three independent experiments. n = 4 or 5/group. Graphical data show mean ± SE, by two-way ANOVA (B–F) and unpaired t test (I–K). *, P < 0.05; **, P < 0.01. Eos, eosinophil.