Subcellular localization of Gimap5 in sorted liver endothelial cells (LECs; DAPI⁻CD45⁻CD31⁺). (A and B) Confocal microscopy of isolated LECs from Gimap5^sph/+ (A) and Gimap5^sph/sph (B) and stained for GIMAP5 (green), lysosomal marker Lamp-1 (red), and mitochondrial marker cytochrome-c (magenta) and counterstained with DAPI (blue). Experimental data were verified in at least two independent experiments. Scale bars = 5 µm. Images shown in Fig. 3 C are also included in the panels above.