Liver pathology of Gimap5^sph/+Rag1^−/− and Gimap5^sph/sphRag1^−/− mice. (A) Gross liver morphology of adult Gimap5^sph/+Rag1^−/− (smooth) and Gimap5^sph/sphRag1^−/− mice (nodular). (B) Reticulin and trichrome stains of liver sections from Gimap5^sph/+Rag1^−/− (left panels) and Gimap5^sph/sphRag1^−/− (right panels) show two-cell-thick plate and increased collagen (blue) deposition solely in mutant mice. (C) Flow cytometric analysis shows increased CD34 expression in liver endothelial cells (DAPI⁻CD45⁻CD31⁺) isolated from Gimap5^sph/sphRag1^−/− mice as compared with Gimap5^sph/+Rag1^−/− mice. Experimental data were verified in at least two independent experiments, and littermates were used as controls. Scale bars = 50 µm.