Mass spectrometric analysis of S1PR1 binding partners.(A–E) HUVECs expressing vector or GFP-tagged S1PR1, Y143D-S1PR1, or Y143F-S1PR1 for 24 h were either left unstimulated (A, D, and E) or stimulated with 1 μM S1P for 5 and 15 min (B and C). Lysates were immunoprecipitated using an anti-GFP antibody. Complexes were separated by SDS-PAGE and analyzed by mass spectroscopy. A scatter plot of mean intensity versus spectral counts is shown. Peptides that were overlapping with GFP were excluded from the mass spectrometry data (see Materials and methods).
Figure S3.

Mass spectrometric analysis of S1PR1 binding partners. (A–E) HUVECs expressing vector or GFP-tagged S1PR1, Y143D-S1PR1, or Y143F-S1PR1 for 24 h were either left unstimulated (A, D, and E) or stimulated with 1 μM S1P for 5 and 15 min (B and C). Lysates were immunoprecipitated using an anti-GFP antibody. Complexes were separated by SDS-PAGE and analyzed by mass spectroscopy. A scatter plot of mean intensity versus spectral counts is shown. Peptides that were overlapping with GFP were excluded from the mass spectrometry data (see Materials and methods).

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