53BP1 regulates IR-induced accumulation of pT308 AKT on the centrosomes. (A and B) Control or si53BP1-treated G1 RPE-1 (A) or HeLa (B) cells were treated with 5 Gy IR. Cells were collected at indicated time points after IR and separated into cytoplasmic (Cyto) and nuclear (Nuc) fractions. Indicated proteins were analyzed by immunoblotting. (C) Upper: pT308 AKT colocalized with NEDD1, and the signal was increased after IR treatment in G1 RPE-1 cells. Lower: 53BP1 depletion inhibited the increase of pT308 after IR. Scale bar, 20 µm for labeled panels, 2 µm for magnified images. (D) Quantitation of the relative pT308 signal intensity at the centrosomes in NC or si53BP1 treated cells is shown in box plot (n > 50). **, P < 0.01; ns, not significant. A.U., arbitrary units; UT, untreated.
Figure 8.

53BP1 regulates IR-induced accumulation of pT308 AKT on the centrosomes. (A and B) Control or si53BP1-treated G1 RPE-1 (A) or HeLa (B) cells were treated with 5 Gy IR. Cells were collected at indicated time points after IR and separated into cytoplasmic (Cyto) and nuclear (Nuc) fractions. Indicated proteins were analyzed by immunoblotting. (C) Upper: pT308 AKT colocalized with NEDD1, and the signal was increased after IR treatment in G1 RPE-1 cells. Lower: 53BP1 depletion inhibited the increase of pT308 after IR. Scale bar, 20 µm for labeled panels, 2 µm for magnified images. (D) Quantitation of the relative pT308 signal intensity at the centrosomes in NC or si53BP1 treated cells is shown in box plot (n > 50). **, P < 0.01; ns, not significant. A.U., arbitrary units; UT, untreated.

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