DMSR led to accumulation of pT308 AKT on the centrosome. (A) Upper: pT308 AKT colocalized with NEDD1 and increased after IR in G1 cells. Lower: DNA-PKcs inhibitor treatment inhibited the increase of pT308 on the centrosome after IR. (B) Box plot shows quantitation of the relative pT308 signal intensity at the centrosomes as assayed in A (n > 50). (C) Upper: pT308 AKT colocalized with NEDD1 and the signal was increased after IR in G1 RPE1 cells. Middle: PDK1 inhibitor (BX-912) did not affect the IR-induced increase of pT308 AKT on the centrosomes. Lower: Cotreatment of DNA-PKcs inhibitor and PDK1 inhibitor (BX-912) inhibit the increase of pT308 after IR. (D) Quantitation of the relative pT308 signal intensity at the centrosome after indicated treatment is shown in box plot (n > 50). Scale bar, 20 µm for labeled panels, 2 µm for magnified images. **, P < 0.01; ns, not significant. A.U., arbitrary units; UT, untreated.
Figure 7.

DMSR led to accumulation of pT308 AKT on the centrosome. (A) Upper: pT308 AKT colocalized with NEDD1 and increased after IR in G1 cells. Lower: DNA-PKcs inhibitor treatment inhibited the increase of pT308 on the centrosome after IR. (B) Box plot shows quantitation of the relative pT308 signal intensity at the centrosomes as assayed in A (n > 50). (C) Upper: pT308 AKT colocalized with NEDD1 and the signal was increased after IR in G1 RPE1 cells. Middle: PDK1 inhibitor (BX-912) did not affect the IR-induced increase of pT308 AKT on the centrosomes. Lower: Cotreatment of DNA-PKcs inhibitor and PDK1 inhibitor (BX-912) inhibit the increase of pT308 after IR. (D) Quantitation of the relative pT308 signal intensity at the centrosome after indicated treatment is shown in box plot (n > 50). Scale bar, 20 µm for labeled panels, 2 µm for magnified images. **, P < 0.01; ns, not significant. A.U., arbitrary units; UT, untreated.

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