CaV1-expression in neurons of cKO mice or after AAV-mediated knock down of Ca _V 1.2. (A) Single-cell data and mean intensities obtained from Ctrl and cKO mice using a Ca_V1-specific antibody (clone L57/46; n = 10 cultures from three mice per genotype, >6,000 neurons per genotype, Student’s t test). (B and C) Single-cell data and mean intensities obtained using a Ca_V1-specific antibody (clone L57/46) at 8 div (B, n = 4, >4,000 neurons per condition) and 15 div (C, n = 4, >1,500 neurons per condition) after transduction with AAV-PHP.S expressing either a scrambled (Scr) or Cacna1c-specific shRNA, indicating down-regulation of Ca_V1 in GFP⁺ neurons. The primary antibody was omitted in respective controls (w/o AB).