Differential gene expression analysis of the neonatal lung ILC2 states defined by trajectory analysis. (A) Heatmap of the expression of ILC2-associated genes in the indicated states. (B) Heatmap shows the genes that are differentially expressed (P ≤ 0.01) among the indicated states. (C) Changes in the expression of the indicated genes along cell fate progression in pseudotime, from state 1 to states 4 and 5. (D) Heatmap shows the expression our selection of ILC(2)-associated genes by neonatal ILCPs and proinflammatory and tissue-repairing ILC2s in Fig. 8 D. The differentially expressed genes are shown in green (higher expression on ILCPs), red (higher expression on proinflammatory ILC2s), and purple (higher expression on tissue-repairing ILC2s) boxes. The rest of the genes are similarly expressed by the two subsets.