YFP expression in lymphoid populations of lung, BM, and intestine of RORα-YFP mice. (A) Lung ILC2s from naive and IL-33–treated adult as well as neonatal (12-d-old) mice were sequentially gated by Lin⁻CD127⁺Thy1⁺ST2⁺CD25⁺, and their expression of YFP in RORα-YFP (black line) and B6 control (filled gray) mice is shown. (B–E) YFP expression by adult lung CD19⁺ B cells and TCRαβ/γδ⁺ T cells (B), TCRαβ/γδ⁻NKp46⁺ (YFP⁺TCRαβ/γδ⁻NKp46⁺ are analyzed for Eomes and T-bet expression; C), BM Lin⁻Thy1⁺CD127⁺PD-1⁺α4β7⁺CD25⁻ ILCPs and Lin⁻CD127⁺Thy1⁺ST2⁺ ILC2Ps (D), and intestinal Lin⁻RORγ⁻GATA-3⁺ ILC2s and Lin⁻RORγt⁺GATA-3^int ILC3s (E) in RORα-YFP (black line) and B6 control (filled gray) mice is shown.