ILCs in RORα-YFP mice express YFP. (A) Lung ILC2s from naive and IL-33–treated adult as well as neonatal (12-d-old) mice were sequentially gated by Lin−GATA-3+ST2+Thy1+, and their expression of YFP in RORα-YFP (black line) and B6 control (filled gray) mice is shown. (B) Lin−YFP+ cells from adult and neonatal lungs as well as adult small intestine were gated and analyzed for the expression of GATA-3 and RORγt as well as GATA-3 and Thy1. Lung Lin−YFP+Thy1+ cells were further analyzed for the expression of ST2 and CD25. Data are representative of three or more independent experiments with three or more mice per group in each experiment.
Figure 1.

ILCs in RORα-YFP mice express YFP. (A) Lung ILC2s from naive and IL-33–treated adult as well as neonatal (12-d-old) mice were sequentially gated by Lin−GATA-3+ST2+Thy1+, and their expression of YFP in RORα-YFP (black line) and B6 control (filled gray) mice is shown. (B) Lin−YFP+ cells from adult and neonatal lungs as well as adult small intestine were gated and analyzed for the expression of GATA-3 and RORγt as well as GATA-3 and Thy1. Lung Lin−YFP+Thy1+ cells were further analyzed for the expression of ST2 and CD25. Data are representative of three or more independent experiments with three or more mice per group in each experiment.

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