dMtmr6 influences PI(3)P reporter distribution. (A, B, G, and H) Representative images of indicated regions of Drosophila fat body overexpressing the PI(3)P reporter GFP-2xFYVE from fed (A and B) and starved animals (G and H). (C–F) Quantification of GFP-2xFYVE puncta from regions of interest as depicted in A″, B″, G″, and H″ (perinuclear) and A″″, B″″, G″″, and H″″ (aponuclear). (I, K, L, and M) Representative images of fed and starved Drosophila fat body overexpressing GFP-2xFYVE, stained with Hoechst to detect nuclei, and antibody stained to detect Atg8a. (J) Quantification of colocalization between GFP-2xFYVE and Atg8a as represented in I and K–M. Data are presented as mean ± minimum to maximum (n ≥ 10; C–F and J). Asterisks denote statistical significance (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001), using one-way ANOVA. Scale bars, 20 µm. n.s., not significant.