dMtmr6 depletion impairs endocytosis and phagocytosis. (A) Representative image of TR-avidin (red)-treated fat body from fed L3 Drosophila larvae with WT control (GFP-negative) and clonal dMtmr6-knockdown (green) cells and TR-avidin only depicted in A′. Tissues were fixed following ex vivo incubation with TR-avidin as described in Materials and methods. (B) Quantification of percent area (μm²) of TR-avidin in WT versus dMtmr6-knockdown cells as represented in A. (C) Control Drosophila macrophage expressing GFP-labeled F-actin (top panels), which contain phagocytic vacuoles (arrowheads) during developmental apoptotic corpse clearance in embryos, compared with dMtmr6-knockdown Drosophila macrophages expressing GFP-labeled actin (bottom panels). (D) Quantification of the number of phagocytic vacuoles in macrophages over time from control versus dMtmr6-knockdown cells. Data are presented as mean ± minimum to maximum (n = 10 paired WT and dMtmr6-knockdown cells taken across random images from three experimental replicates; B) and mean ± SD (n ≥ 11; C). Asterisks denote statistical significance (****, P < 0.0001), using paired two-tailed t test. Scale bars represent 20 µm (A), 20 µm (C, first panel from left), and 10 µm (C, second panel from left).