Figure 1.
Drosophila CG3530/dMtmr6 negatively regulates Atg8a puncta formation.(A) Control (GFP-negative) and dMtmr6-knockdown cells (white dotted line, green) from midguts expressing pmCherry-Atg8a during the feeding L3 stage. (B) Quantification of pmCherry-Atg8a puncta levels in WT control cells and dMtmr6-knockdown cells of midguts from feeding L3 and 2 h APF pupae. (C) Control (GFP-negative) and dMtmr6-knockdown cells (white dotted line, green) from midguts expressing pmCherry-Atg8a during developmental autophagy (bottom) occurring 2 h APF. (D) Quantification of pmCherry-Atg8a puncta size in WT and dMtmr6-knockdown cells from 2 h APF midguts. (E) Larval Drosophila fat body with control (GFP-negative) and dMtmr6-knockdown cells (white dotted line, green) stained with an antibody to detect Atg8a and Hoechst to detect nuclei (blue) from fed animals. (F) Quantification of percentage of cellular area occupied by Atg8a puncta in WT and dMtmr6-knockdown cells of L3 fat body from fed or starved animals as represented in E and G. (G) Larval Drosophila fat body with control (GFP-negative) and dMtmr6-knockdown cells (white dotted line, green) stained with an antibody to detect Atg8a and Hoechst to detect nuclei (blue) from starved animals. (H) Quantification of Atg8a puncta size in WT and dMtmr6-knockdown cells of L3 fat body from fed or starved animals as represented in E and G. Data in B, D, F, and H are presented as mean ± minimum to maximum (n ≥ 10). Asterisks denote statistical significance (***, P < 0.001; ****, P < 0.0001) using paired, two-tailed t test. Scale bars, 20 µm.

Drosophila CG3530/dMtmr6 negatively regulates Atg8a puncta formation. (A) Control (GFP-negative) and dMtmr6-knockdown cells (white dotted line, green) from midguts expressing pmCherry-Atg8a during the feeding L3 stage. (B) Quantification of pmCherry-Atg8a puncta levels in WT control cells and dMtmr6-knockdown cells of midguts from feeding L3 and 2 h APF pupae. (C) Control (GFP-negative) and dMtmr6-knockdown cells (white dotted line, green) from midguts expressing pmCherry-Atg8a during developmental autophagy (bottom) occurring 2 h APF. (D) Quantification of pmCherry-Atg8a puncta size in WT and dMtmr6-knockdown cells from 2 h APF midguts. (E) Larval Drosophila fat body with control (GFP-negative) and dMtmr6-knockdown cells (white dotted line, green) stained with an antibody to detect Atg8a and Hoechst to detect nuclei (blue) from fed animals. (F) Quantification of percentage of cellular area occupied by Atg8a puncta in WT and dMtmr6-knockdown cells of L3 fat body from fed or starved animals as represented in E and G. (G) Larval Drosophila fat body with control (GFP-negative) and dMtmr6-knockdown cells (white dotted line, green) stained with an antibody to detect Atg8a and Hoechst to detect nuclei (blue) from starved animals. (H) Quantification of Atg8a puncta size in WT and dMtmr6-knockdown cells of L3 fat body from fed or starved animals as represented in E and G. Data in B, D, F, and H are presented as mean ± minimum to maximum (n ≥ 10). Asterisks denote statistical significance (***, P < 0.001; ****, P < 0.0001) using paired, two-tailed t test. Scale bars, 20 µm.

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