Quantification of VIF distribution in the perinuclear region. (a and b) Examples of polar histogram plots of a moving average of fluorescence intensity at 1-µm intervals around the nucleus of a vim^+/+ (a) and a rescued (b) mEF. Distance from the origin indicates averaged fluorescence intensity, while the angle of the intensity is indicated in degrees. The thick blue line indicates the fluorescence intensity at a distance of 1 µm from the nucleus. Other lines indicate fluorescence intensity at the distances corresponding to the color bar. (c–f) Examples of the outer boundaries of the ring-like regions around the nucleus. (c and d) The observed vimentin immunofluorescence in green from confocal imaging of the vim^+/+ and rescued cells, respectively. The blue text indicates the circular variance of the vimentin immunofluorescence intensity bounded by the thick blue line. (e and f) Merges in the lamin stain of the nucleus in magenta. (g) The distribution of circular variance in the vim^+/+ and rescue cells using box plots (the number of samples from a single pair of experiments is shown at the bottom: wild-type, n = 27; rescue, n = 77). The thick horizontal line indicates the location of the median. The lower and upper parts of the box represent the 25th and 75th percentiles. The ends of the notch indicate 1.57 times the interquartile range divided by the square root of the number of samples. The whiskers indicate the range of the circular variance up to 1.5 times the interquartile range away from the quartiles. Scale bar is 20 µm.