Overall distribution of VIF in vim^+/+, vim^−/−, and rescued mEFs. (a–c) Immunofluorescence (maximum-intensity projection of confocal stacks)/phase-contrast images of vimentin (green) in vim^+/+ (a), vim^−/− (b), and rescued mEFs expressing exogenous wild-type vimentin (c). (d and e) SIM images of the vimentin cage around the nucleus (magenta) in a rescued mEF at its basal (d) and apical (e) planes. (f) Maximum projection of the SIM images for the cell in d and e. Scale bar is 20 µm.