Perinuclear cage of VIF. (a–c) Confocal images of the VIFs (green) at the lower (a) and upper (b) surfaces of the nuclei (magenta) and their 3D rendering (c) in two vim^+/+ mEFs. (d and e) SIM images of the vimentin cage around the nucleus (magenta) in a vim^+/+ mEF at the basal (d) and apical (e) surfaces of the nucleus. (f) Maximum projection of SIM images for the cell in d and e. Scale bar is 20 µm.