IMC reveals that CD16⁺CD163⁺ monocytes are adjacent to blood vessels in NEC mucosa. Composite of surface markers (individual layers) of interest generated from MCD files using histocat 1.7.6.1 (Fluidigm) from representative neonatal and NEC samples, White scale bar, 50 μm. Gray scale bar, 100 μm. White asterisk denotes one CD16⁺CD163⁺ monocyte. DNA, 191Ir DNA.