BM CD138⁺NIP⁺IgG1⁺ cells display elevated levels of the integrins α4 and β1 on their surface compared with splenic CD138⁺NIP⁺IgG1⁺ cells from the same animal. (A and B) Plots showing geometric mean fluorescent intensities (gMFIs) for surface integrin α4 (A) and β1 (B) signals on the population of CD138⁺NIP⁺IgG1⁺ cells from the spleens of Zfp36l1^fl/fl CD79a^+/+ mice at days 14 and 42. The staining and analysis were performed on the same day with the same flow cytometer settings; the data for two independent experiments are shown (n ≥ 3 per experiment; two-way ANOVA on logarithmically transformed data; the P values shown are for the day factor). (C and D) Plots showing gMFI for surface integrin α4 (C) and β1 (D) signals on the population of CD138⁺NIP⁺IgG1⁺ cells from the spleens and BM of individual Zfp36l1^fl/fl CD79a^+/+ (closed circles) and Zfp36l1^fl/fl CD79a^Cre/+ (open squares) mice analyzed at day 42 (n ≥ 4; two-way ANOVA; the P values shown are for the organ factor). (E–G) Plots showing percentages of cells with a high α4β7 surface abundance (F) and gMFI for surface level of the integrin chains β1 (E) and α4 (G) among both EdU-high NP-specific ASCs and EdU-low NP-specific ASCs in the BM of mice of both genotypes at day 4 after reimmunization. Populations were identified as depicted in Fig. S5 F (n = 3; two-way ANOVA; the P value shown is for the factor “type of ASC”). (H–J) Plots showing gMFIs for CXCR4 (H) and for the surface integrin α4 (I) and β1 (J) signals on the population of CD138⁺NIP⁺IgG1⁺ cells from the spleen and BM of individual Zfp36l1^fl/fl CD79a^+/+ mice at day 14 and day 42 after primary immunization (n ≥ 4 per time point; two-way ANOVA; the P values shown are for the day factor). For all plots in all panels, each symbol represents data from an individual mouse, and lines represent means. Where it is not indicated otherwise, data are representative of at least two independent experiments. Exp, experiment; Spl, spleen; hi, high.