The phenotype of CD4⁺ and CD8⁺ Tconv cells in Bach2^fl/flFoxp3^{EGFP-Cre-ERT2} mice. (A) Frequencies of indicated CD8⁺ T cell subsets within spleens of animals of the indicated genotypes treated with tamoxifen for 8 wk. (B) Representative flow cytometry showing CD44 and CD62L expression on gated CD8⁺ T cells (left) and frequencies of indicated T cell subsets (right) within inguinal lymph nodes of animals of the indicated genotypes treated with tamoxifen for 8 wk. (C) Representative flow cytometry showing CD44 and CD62L expression on gated CD4⁺ T cells (left) and frequencies of indicated T cell subsets (right) within spleens of animals of the indicated genotypes treated with tamoxifen for 8 wk. (D) Frequency of ICOS-expressing CD4⁺ T cells within spleens of animals of the indicated genotypes treated with tamoxifen for 8 wk. (E) Representative flow cytometry of IFN-γ expression by gated CD8⁺ and Foxp3⁻ CD4⁺ T cells from spleens of animals of the indicated genotypes treated with tamoxifen for 8 wk. (F) Frequency of IFN-γ–expressing CD4⁺ T cells from spleens of animals of the indicated genotypes treated with tamoxifen for 8 wk. Data are representative of two independently repeated experiments of nine (A), six to seven (B), six to nine (C), seven (D), and nine (E and F) mice per group. *, P < 0.05; **, P < 0.01; ns, not significant; unpaired two-tailed Student’s t test (A–D and F). Numbers in gates show percentages. Bars and error show mean and SEM.