NKG2D marks proinflammatory Th17 cells in vivo. (A) Schematic representation of the experimental workflow during antigen/adjuvant immunizations. (B and C) Representative FC analysis (B) and quantification of intracellular cytokine expression (C) in transferred OT-II cells gated as NKG2D+ and NKG2D−, or Klrk1−/− OT-II cells, from draining lymph nodes of recipient mice at day 6 after immunization with OVA323–339/CFA and after restimulation with PMA/Iono. Each symbol represents a mouse. Line represents mean ± SEM (n = 7), Mann–Whitney U test. Data are pooled from two independent experiments.
Figure 6.

NKG2D marks proinflammatory Th17 cells in vivo. (A) Schematic representation of the experimental workflow during antigen/adjuvant immunizations. (B and C) Representative FC analysis (B) and quantification of intracellular cytokine expression (C) in transferred OT-II cells gated as NKG2D+ and NKG2D−, or Klrk1−/− OT-II cells, from draining lymph nodes of recipient mice at day 6 after immunization with OVA323–339/CFA and after restimulation with PMA/Iono. Each symbol represents a mouse. Line represents mean ± SEM (n = 7), Mann–Whitney U test. Data are pooled from two independent experiments.

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