Figure S2.
Notch1 and CXCR4 polarize at the interface between DN3a and OP9-DL1 stromal cells. DN3 cells were incubated with OP9-DL1 cells for 14 h and then fixed and stained for α-tubulin to mark the MTOC and either Notch1 or CXCR4 (as shown). Maximum projection of z-stack images were acquired using confocal microscopy, and representative images are shown. After triaging for cells in which the MTOC was recruited to the interface (indicated by asterisks) with an OP9-DL1 cell, the percentage of cells in which Notch1 and CXCR4 were polarized to the interface with the OP9-DL1 cell was determined by blind scoring (as shown by column-bar plot). The total number of scored conjugates per marker is 75 (25 cells per biological replicate). Scale bar, 5 µm. Error bars represent SEM.

Notch1 and CXCR4 polarize at the interface between DN3a and OP9-DL1 stromal cells. DN3 cells were incubated with OP9-DL1 cells for 14 h and then fixed and stained for α-tubulin to mark the MTOC and either Notch1 or CXCR4 (as shown). Maximum projection of z-stack images were acquired using confocal microscopy, and representative images are shown. After triaging for cells in which the MTOC was recruited to the interface (indicated by asterisks) with an OP9-DL1 cell, the percentage of cells in which Notch1 and CXCR4 were polarized to the interface with the OP9-DL1 cell was determined by blind scoring (as shown by column-bar plot). The total number of scored conjugates per marker is 75 (25 cells per biological replicate). Scale bar, 5 µm. Error bars represent SEM.

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