Potential phosphorylation sites involved in the ACBD4/5–V APB interaction. (A) Schematic overview of ACBD4 and ACBD5 domain structure, including the amino acid sequences of the FFAT-like motif region, with the phosphorylation sites mutated in this study in bold. (B) Alignment of the FFAT-like motif region of ACBD4 and ACBD5 from Hs, Homo sapiens (human); Rn, Rattus norvegicus (rat); Mm, Mus musculus (mouse); Cl, Canis lupus familiaris (dog); Fp, Falco peregrinus (falcon); Xt, Xenopus tropicalis (frog); and Dr, Danio rerio (zebrafish). Conservation of the phosphorylation sites reported in this study is indicated in bold. (C) Overview of the FFAT-like motif region of ACBD4 and ACBD5 and the constructs used in this study. Mutated residues are indicated in bold.