Targeted RNAi screen identifies endosomal genes involved in SG maturation. (A–F) Spinning-disc confocal images of live L3 salivary gland cells expressing Sgs3-DsRed (grayscale). (A) Examples of SG RNAi phenotypes scored on a scale from 0 (normal) to 4 (severe). (B–F) Examples of RNAi knockdowns showing defects in SG maturation. (G) List of positive hits from the endocytic pathway.