Dynein inhibition increases membrane expression of Kv7.4 protein. (A, i–iii) Representative midcell z-section of a mesenteric artery myocyte treated with or without ciliobrevin D and stained for Kv7.4 (top) or NCX (bottom; i). Scale bars, 10 μm. Mean membrane intensity of Kv7.4 (n = 9; ii) and NCX (n = 10 or 11; iii) relative to total intensity in cells treated with ciliobrevin D compared with nontreated cells, calculated from midcell z section. Significance was determined by an unpaired t test. **, P < 0.001. (B i) Representative midcell z section of HEK293B cells transfected with Kv7.4-EGFP (green) in nontreated and ciliobrevin D– or colchicine-treated cells. The nuclei are stained with 4′,6-diamidino-2-phenylindole (blue). Scale bars, 10 μm. (B ii) Mean membrane intensity of Kv7.4 relative to total intensity in cells treated with ciliobrevin D (n = 5) or colchicine (n = 7) compared with nontreated cells (n = 6), calculated from midcell z section. Significance was determined by a one-way ANOVA. **, P < 0.001; ***, P < 0.0001. Error bars show mean and SEM.