Kv7 channel function is enhanced following dynein inhibition. (A) Representative isometric tension recordings of rat mesenteric artery segments preconstricted with methoxamine (•) before sequentially increasing concentrations of Kv7.2–Kv7.5–specific activator NS15370 were applied in control (left) and ciliobrevin D–treated (right) arteries. (B) Mean concentration-effect curves and EC₅₀ values to the Kv7.2–Kv7.5–specific activator NS15370 showing the effect of NS15370 in rat mesenteric artery segments before and after 10 µM ciliobrevin D incubation. Mean EC₅₀ values were compared according to an unpaired t test. **, P < 0.01. (C) 10 µM ciliobrevin D enhanced the relaxation to another Kv7.2–Kv7.5 channel activator, S-1, according to an unpaired t test. *, P < 0.05. (D) Rat mesenteric arteries were transfected with either a Kv7.4-targeted or the control (miss-match) morpholino. Relaxations to S-1 were inhibited in arteries transfected with the Kv7.4-targeted morpholino compared with arteries transfected with the control morpholino. Ciliobrevin D enhanced relaxations to S-1 in control arteries, but ciliobrevin D was unable to enhance S-1–mediated relaxation in Kv7.4 knockdown arteries. A one-way ANOVA followed by a Sidak multiple comparisons test was performed, with ** and *** denoting P < 0.01 and P < 0.001, respectively. Error bars show the mean and SEM.