Figure 9.
Examination of slow recovery from inactivation in a fraction of FGF14 KO cells.(A and B) Recovery from inactivation in WT (A) and FGF14 KO (B) cells was compared for recovery at −80 mV from a standard single step (1P) to 0 mV, and then for recovery following a 10-Hz train of 10 steps to 0 mV (10P). (A) For 22 WT cells, recovery following a 10-Hz train results in a use-dependent decrease in the fast recovery component. For 1P, Af = 0.47, τf = 6.03 ms, As = 0.53, τs = 549.4 ms. For 10P, Af = 0.20, τf = 7.35 ms, As = 0.77, τs = 589.5 ms. (B) For averages of 19 FGF14 KO cells compared with 1P and 10P protocols, the 1P protocol primarily results in exclusively a fast recovery component, but some reduction in the fast component can occur in the 10P protocol (see Fig. S2). Note the large standard error associated with the 10P recoveries. For the 1P protocol, a single-exponential fit yielded: Af = 0.95 ± 0.01, τf = 7.5 ± 0.9 ms; while, for a two-exponential fit, Af = 0.94 ± 0.02, τf = 6.9 ± 0.5 ms, As = 0.05 ± 0.07, τs = 1,090.1 ±3,892.3 ms. For the 10P protocol, the two-exponential fit yielded Af = 0.79 ± 0.01, τf = 7.4 ± 0.5 ms, As = 0.20 ± 0.1, τs = 1,664.0 ± 1,807.2 ms. Note the large confidence limits reflecting the parameters describing the slow component, given that the data do not strongly define this component. (C) Recoveries following the 10P protocol for 22 individual WT cells are plotted with the three cells with the largest fast recovery component highlighted in green. (D) Recovery following the 10P protocol is plotted for 19 FGF14 KO cells, with the 3 cells showing the most markedly reduced fast recovery highlighted in red. (E) Peak current diminution for the 22 WT cells during the 10P protocol are plotted. Green highlights cells in C with a larger component of fast recovery. (F) Peak current diminution during the 10-Hz train is plotted for the 19 FGF14 KO cells. For cells with exclusively fast recovery from inactivation (blue), there is little peak current diminution at 10 Hz. (G) The fraction of residual fast component (Af) following the 10P protocol is plotted as a function of the peak Nav current diminution during the 10P train for WT (blue; green) and FGF14 (red) cells.

Examination of slow recovery from inactivation in a fraction of FGF14 KO cells. (A and B) Recovery from inactivation in WT (A) and FGF14 KO (B) cells was compared for recovery at −80 mV from a standard single step (1P) to 0 mV, and then for recovery following a 10-Hz train of 10 steps to 0 mV (10P). (A) For 22 WT cells, recovery following a 10-Hz train results in a use-dependent decrease in the fast recovery component. For 1P, Af = 0.47, τf = 6.03 ms, As = 0.53, τs = 549.4 ms. For 10P, Af = 0.20, τf = 7.35 ms, As = 0.77, τs = 589.5 ms. (B) For averages of 19 FGF14 KO cells compared with 1P and 10P protocols, the 1P protocol primarily results in exclusively a fast recovery component, but some reduction in the fast component can occur in the 10P protocol (see Fig. S2). Note the large standard error associated with the 10P recoveries. For the 1P protocol, a single-exponential fit yielded: Af = 0.95 ± 0.01, τf = 7.5 ± 0.9 ms; while, for a two-exponential fit, Af = 0.94 ± 0.02, τf = 6.9 ± 0.5 ms, As = 0.05 ± 0.07, τs = 1,090.1 ±3,892.3 ms. For the 10P protocol, the two-exponential fit yielded Af = 0.79 ± 0.01, τf = 7.4 ± 0.5 ms, As = 0.20 ± 0.1, τs = 1,664.0 ± 1,807.2 ms. Note the large confidence limits reflecting the parameters describing the slow component, given that the data do not strongly define this component. (C) Recoveries following the 10P protocol for 22 individual WT cells are plotted with the three cells with the largest fast recovery component highlighted in green. (D) Recovery following the 10P protocol is plotted for 19 FGF14 KO cells, with the 3 cells showing the most markedly reduced fast recovery highlighted in red. (E) Peak current diminution for the 22 WT cells during the 10P protocol are plotted. Green highlights cells in C with a larger component of fast recovery. (F) Peak current diminution during the 10-Hz train is plotted for the 19 FGF14 KO cells. For cells with exclusively fast recovery from inactivation (blue), there is little peak current diminution at 10 Hz. (G) The fraction of residual fast component (Af) following the 10P protocol is plotted as a function of the peak Nav current diminution during the 10P train for WT (blue; green) and FGF14 (red) cells.

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